Reduction of endogenous nucleic acid in a single-cell protein

A Single-stranded Nucleic Acid-binding Protein from Artemia salina

A protein that binds tightly to single-stranded but not to double-strained nucleic acids has been purified to homogeneity from a high salt wash of ribosomes from cryptobiotic Artemia salina gastrulae. The protein, designated HD40 to indicate a helix-destabilizing protein with a molecular weight of 40,000, is present in the high-salt ribosomal wash at a level of about 2 molecules per 80 S riboso...

Evaluation of Single Cell Protein as a Non-Conventional Feedstuff in Broilers Feeding

Single cell protein (SCP) is the definition for dried cells originated from single-celled organisms intended to be used as a protein source in human foods or animal feeds. An experiment has been conducted to evaluate the various levels of single cell protein on fecal microflora and productive traits of broiler chickens. A total of 192 day-old Ross 308 male chicks were randomly divided into 4 tr...

Protein–nucleic acid interactions

Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation

A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon ...

Droplet microfluidics for single cell and nucleic acid analysis

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